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Agarose Gel Electrophoresis

Addgene
26.1K subscribers
294K views 7 years ago
Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and purification. In this video, we’ll show you how to prepare an agarose gel using powdered agarose and buffer. Then we’ll show you how to load your samples into the gel and perform electrophoresis to successfully separate your DNA bands. We’ll also give you some tips and tricks to get great looking gels every time! For the full written Agarose Gel Electrophoresis protocol, visit: https://www.addgene.org/protocols/gel... ___________________________________________________________________________________________________ 0:00 Intro 0:49 What Percent Agarose to Use? 1:28 Preparing the Gel 3:04 Pouring the Gel 3:34 Loading the Samples and Running an Agarose Gel ...more
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Agarose Gel Electrophoresis
2.6KLikes
294,334Views
2017Dec 11
Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and purification. In this video, we’ll show you how to prepare an agarose gel using powdered agarose and buffer. Then we’ll show you how to load your samples into the gel and perform electrophoresis to successfully separate your DNA bands. We’ll also give you some tips and tricks to get great looking gels every time! For the full written Agarose Gel Electrophoresis protocol, visit: https://www.addgene.org/protocols/gel... ___________________________________________________________________________________________________ 0:00 Intro 0:49 What Percent Agarose to Use? 1:28 Preparing the Gel 3:04 Pouring the Gel 3:34 Loading the Samples and Running an Agarose Gel

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Addgene

26.1K subscribers

Protocols

Addgene
16 / 22
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Coomassie Purity Staining of Recombinant Antibodies - Addgene Protocol

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Recombinant Antibody Affinity Purification with Protein A or Protein G Protocol

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Western Blot Protocol

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How to Design Primers for PCR

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Polymerase Chain Reaction (PCR) Protocol

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6

Creating Bacterial Glycerol Stocks

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7

Counting Cells

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8

Purifying DNA from an Agarose Gel

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9

AAV Titration by qPCR

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10

Sterile Technique

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11

Getting Started with Tissue Culture

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12

Inoculating Liquid Bacterial Culture

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13

Bacterial Transformations

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14

Multichannel Pipetting Technique

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15

AAV Purification

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▶

Agarose Gel Electrophoresis

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17

Over-Agar Antibiotic Plating

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18

Streaking for Single Colonies

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19

Restriction Digest Analysis

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Plate Pouring Protocol

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Storing and Handling of your Addgene Plasmids

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22

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

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